The early mammalian embryo traverses remarkably different environments before specification of somatic cell lineages occurs during gastrulation. Transitioning through different states of pluripotency is thought to provide cells a method of accommodating different conditions while they expand in numbers during the embryo’s journey. I will present some of our efforts to elucidate the mechanisms stem cells use to transition between states of pluripotency. I will focus on recent discoveries made using a CRISPR-Cas9 genome wide screening platform, which identified some frankly unexpected and robust effects from genes important for nuclear export and homeostasis of intracellular calcium. In addition, I will present some of our efforts towards improving CRISPR genome editing through improving our understanding of how the bacterial Cas9 protein interfaces with the mammalian genome when we attempt to edit genomes.
Brad Merrill, Ph.D.
Associate Professor, Department of Biochemistry and Molecular Genetics
Director, Genome Editing Core
University of Illinois at Chicago
12:00 pm at Van Andel Institute
Conference Room 3104/3105
For questions, please contact Kim Cousineau at 616.234.5684.