Cryopreservation Method

Sperm is isolated from the cauda epididymis and vas deferens of the male mice into warmed cryoprotective medium supplemented with an anti-oxidant. Sperm is allowed to “swim-out” for 10 minutes, and then 4, 1 ml aliquots are aspirated into each straw. Approximately 20 straws are used. Each straw is heat sealed and placed into a cassette (5 straws/cassette). Loaded cassettes are statically frozen in liquid nitrogen vapor for 10 minutes, then plunged into liquid nitrogen and moved to two different liquid nitrogen tanks for long-term storage. Triple banking offsite is an option for an additional cost.