DNA Construct Preparation

1. Using appropriate enzymes, digest the transgenic DNA construct to excise the transgene from the vector.
2. Electrophorese a small amount of the digest on a gel to make sure the digestion is complete and to photograph the gel.
3. Include with your application, a picture of the gel showing correct digestion and clearly indicating which band contains the construct.
4. We require at least 10 ug of the digested plasmid since there is loss during the purification steps.
5. Send the digest to us without further purification or as an ethanol precipitate.
6. Once the construct is received, it will be gel electrophoresed, electroeluted from the excised gel fragment, purified through an Elutip column, and ultimately resuspended in a transgenic buffer containing embryo-tested water.