Our Research
Primers
We have various standard vector-specific primers and a set of primers for sequencing out of common vectors. We encourage designing primers using software, such as Oligo 
(current version 6.0 for PC and Mac). You can also use freeware such as Web-Primer or PRIDE .
Once ordered, your primers should be diluted to the standard sequencing concentration (1ul = 3.2pmol).
Primers Supplied by SeqCore
| Primer Name |
Sequence |
| U.FORW |
TGTAAAACGACGGCCAGT |
| U.REV |
CAGGAAACAGCTATGACC |
| M13.FORW |
GTAAAACGACGGCCAGT |
| M13.REV |
GGAAACAGCTATGACCATG |
| T3.BS |
AATTAACCCTCACTAAAGGG |
| T7.BS |
GTAATACGACTCACTATAGGGC |
| SK |
CGCTCTAGAACTAGTGGATC |
| KS |
TCGAGGTCGACGGTATC |
| SP6.Gibco |
ATTTAGGTGACACTATAG |
| SP6.PROMEGA |
GATTTAGGTGACACTATAG |
| T7.Gibco |
AACAGCTATGACCATG |
| T7L |
TAATACGACTCACTATAGGG |
| pRSET.REV |
TAGTTATTGCTCAGCGGTGG |
| pGEX |
GGGCTGGCAAGCCACGTTTGGTG |
| bacpacT7 |
GCCGCTAATACGACTCACTATAGGGAGAG |
| bacpacSP6 |
GTTTTTGCGATCTGCCGTTTC |
| Belo295U |
CGACGGCCAGTGAATTGTAAT |
| Belo289U |
GTAAAACGACGGCCAGTGAAT |
| Belo225U |
AAAGGGGGATGTGCTGCAAGG |
| Belo235U |
GTGCTGCAAGGCGATTAAGTT |
Common Vector / Primer Pairs
| Vector |
Primers |
| pUC18 |
U.FORW, U.REV |
| pBluescript |
M13.FORW, M13.REV, T7.BS, T3.BS, SK, KS |
| pRSETA |
T7L, pRSET.REV |
| pGEM |
T7L |
| pcDNA |
T7L |
| pCMV-Sport |
T7L |
| pCRII-TOPO |
M13.FORW, M13.REV |
| pBeloBAC |
Belo295U, Belo289U, Belo225U, Belo235U |
| pPAC4 |
bacpacT7, bacpacSP6 |
| pT7T3D-Pac |
T7L,T3.BS t3, t7, M13.FORW |