Services

Antibody Production: Rabbit polyclonal, and mouse or rat monoclonal antibodies.

Antigen: The antigen to be used for immunization can be the following forms: purified proteins (500 µg–2 mg), nonpurified proteins (1–5 mg), peptides (5–10 mg), DNAs (200–500 µg), and whole cells (1–2 x 10⁷ cells/injection). If the amount of your antigen is limited, please discuss with us first and we will develop a special protocol for your case. If you are going to use DNA as an immunogen, you will be responsible for preparing the construction and testing it in your in vitro system prior to primary immunization.

Immunization: Usually takes 8 to 16 weeks from ordering the animals. Primary immunization is followed by one or several boosters at two- to four-week intervals; the final boost will be given one month after the previous booster.

Serum screening: We will be collecting serum samples beginning with the second booster, testing the reactivity to your antigen by ELISA. The result will be sent to you with a comment as to whether additional immunization is needed or not. A small amount of serum samples will be also available to you so that you may test them using IP, Western, cell stain, etc. For nonpurified proteins, DNAs, and whole cells, you are responsible to provide with us a reasonable screening method. We can discuss this case by case.
Fusion: We will be using splenocytes fused with myeloma cells and placed into 10 x 96 well plates. Usually, about 1,000 to 5,000 first clones of a hybridoma can be obtained. The remaining spleen cells will be frozen and kept in our facility.

Screening: Our standard screening method is ELISA, which means small amount of purified protein is required for coating the EIA plates. If it’s not available, we need to discuss another suitable method.
Recloning: The positive hybridoma cells will be recloned at least twice by limited diluting in order to establish a stable hybridoma cell line.

Characterization: IgG isotyping is standard; IP, Western, and IFA are also available upon request.

Expanding, freezing and growing of hybridoma cell lines: Up to 10 original, different, positive clones and 4 first reclones will be frozen, 2 vials each. The final stable hybridoma cell lines will be frozen, 10 vials each.

Antibody production: Small scale—5 to 10 mg, no additional charge. Large scale—50 mg and up, $50 per milligram.

Purification: All of IgGs (final products) will be purified through a Protein-G affinity column and dialyzed against PBS (or other buffer system). IgG concentration is determined and can be adjusted to proper concentration upon your request.

Lyophilization: Only for large-scale production and upon your request.

Additional services: (with additional charge)

• Immunolabeling 
• Affinity column preparation 
• Bi-specific mAbs 
• Detection methods: sandwich ELISA, immunoblotting, immunofluorescent staining, etc.