Standard Operating Procedures

Direct all questions regarding this information to Sok Kean Khoo, Ph.D.

Sample Preparation

RNA Isolation

1.1  From Cells/etc.
1.1.1   RNeasy
1.1.2   LiCl
1.2  From Tissue
1.2.1    Whole Tissue RNA Isolation
1.2.2    Whole Blood
1.2.3    Blood Spot Cards
1.3  RNA Storage
1.4  RNA Amplification

 

Gene Expression Analysis

Agilent Oligonucleotide Arrays

2.1  Agilent One-Color Microarrays
2.2  Agilent Two-Color Microarrays
2.3  cDNA Microarrays
2.3.1  cDNA Microarray Production
2.3.2  cDNA Microarray Analysis
2.3.3  Direct Labeling

 

aCGH Analysis

3.1  DNA Isolation
3.2  Agilent Two-color arrayCGH

 

miRNA Analysis

LMT.1   One color miRNA Microarrays

 

Hauenstein Parkinson’s Center

4.1  Sample Collection
4.2  SEE (5. VATR)
4.3  Sample Processing
4.3.1  RNA
4.3.2  DNA
4.3.3  Serum, Plasma, and Blood Spot Cards
4.4  Sample Storage

 

Van Andel Tissue Repository (VATR)

5.1  Printing Donor Labels
5.2  Data Entry

 

Reagent Preparation

6.1  cDNA Microarray Solutions
6.2  LMT Reagent LogBook

 

Appendices

7.1  NanoDrop ND1000 Spectrophotometer
7.2  Agilent BioAnalyzer
7.3  Microarray Scanning
7.4  Agilent Feature Extraction
7.5  Assigning Freezer Locations