LMT Protocols

Laboratory of Microarray Technology Standard Operating Procedure

Direct all questions regarding this information to Ken Olinger.

Sample Preparation

• 1.1 RNA Isolation
• 1.1.1 Cell Culture
• 1.1.2 Tissue
• 1.1.2.1 Paired Histology and RNA Isolation
• 1.1.2.2 Whole Tissue RNA Isolation
• 1.1.3 Whole Blood
• 1.1.4 Blood Spot Cards
• 1.2 RNA Storage
• 1.3 RNA Amplification

Gene Expression Analysis

• 2.1 cDNA Microarrays
• 2.1.2 cDNA Microarray Production
• 2.1.4 cDNA Microarray Analysis
• 2.1.4.1 Direct Labeling
• 2.2 Agilent Two-Color Microarrays
• 2.3 Agilent One-Color Microarrays
• 2.4 Agilent Microarray Quality Assurance
• 2.5 cDNA Microarray Quality Control
• 2.6 cDNA Microarray Quality Assurance

Hauenstein Parkinson’s Center

• 3.1 Sample Collection
• 3.2 Sample Processing
• 3.2.1 RNA
• 3.2.2 DNA
• 3.2.3 Serum, Plasma, and Blood Spot Cards
• 3.3 Sample Storage
• 3.4 Exon Sequence Analysis

Van Andel Tissue Repository (VATR)

• 4.1 Printing Donor Labels
• 4.2 Data Entry

Freezer Organization and Inventory

• 5.1 Assigning Freezer Locations

Reagent Preparation

• 6.1 Alcohols
• 6.2 cDNA Microarray Solutions   
• 6.3 Nuclease-Free Water

Appendices

• 7.1 NanoDrop ND1000 Spectrophotometer
• 7.2 Agilent BioAnalyzer
• 7.3 LMT Reagent LogBook
• 7.4 Microarray Scanning
• 7.5 Agilent Feature Extraction