Services

We prepare tissues in accordance with investigator requisitions and instructions (e.g. flat embedding, serial sections, etc) and the standard histopathology procedures that are routine in the pathology community. These can be found in the texts listed below, but are not limited to them. We will accommodate all investigators to the extent possible.

Services we offer include:

• Paraffin Processing
• Frozen Sectioning
• Laser Capture Microdissection (Arturus)
• Vibratome Processing/Sectioning
• Laser Scanning Confocal Imaging (Zeiss 510 Meta)
• Intravital Imaging (CRI Maestro)
• Spectral Imaging and Deconvolution (CRI Nuance)

References and Texts:

• Sheehan, D., and B. Hrapchak, Eds. 1987. Theory and Practice of Histotechnology. Columbus, Ohio: Battelle Press.
• Carson, F. 1996. Histotechnology. Second ed. Chicago: ASCP Press.
• Javois, L. Editor. 1999. Immunocytochemical methods and protocols. In Methods in Molecular Biology, Vol. 115. Totowa, N.J.: Human Press.
• Armed Forces Institute of Pathology, American Registry in Pathology. 1992. Laboratory Methods in Histotechnology. Washington, D.C.
• Resau, J., E. Hudson, and B. Sathyanarayana. 2000. Use of confocal microscopy techniques in the study of transgenic and knockout mice. In Pathology of Genetically Engineered Mice. J. Ward, R. Maronpot, J. Sundberg, and R. Frederickson, Eds. Ames, Iowa: Iowa State University Press. 
• Kane, A.S., R.O. Bennett, J.H. Resau, J.R. Cottrell, R. Reimschuessel, E.B. May, M. Streb, R. Hutchenson, and M.M. Lipsky. 1991. Comparison to cell, tissue, and whole organism bioassay to asses inorganic metal toxicity in the aquatic environment. In Alternative Methods in Toxicology, Vol 8: In Vitro Toxicology- Mechanisms and New Technology. New York: Marcel Dekker.