Trisomy 8 Screen

Trisomy 8 is the most common chromosomal abnormality found in mouse embryonic stem (ES) cell lines. ES cells with three copies of chromosome 8 rarely produce germline transmission. We have developed a “speedy” FISH technique to screen for trisomy 8 in positive clones of mouse ES cells to avoid the use of abnormal cells in generating gene-targeted mice. This step reduces the time and cost of generating mouse model chimeras.

 Investigator responsibilities: 

1. Provide fixed, frozen, or actively growing mouse ES cells. 
2. Complete a project application and CTA agreement form.