Spectral Karyotyping (SKY)

SKY is a powerful 24-color, whole-chromosome painting assay that allows the simultaneous visualization of each chromosome in different colors. Five spectrally distinct dyes are used in combination to create a cocktail of probes unique to each chromosome. The probe mixture is hybridized to metaphase chromosomes on a slide and then visualized with a spectral interferogram cube, which allows the measurement of the entire emission spectrum with a single exposure. The image is processed by computer software that can distinguish differences in color not discernible to the naked eye by assigning a numerical value to the RGB and pseudo color images.

SKY can detect chromosomal material of unknown origin, complex rearrangements, translocations, large deletions, duplications, aneuploidy, and more. Some disadvantages of SKY include ineffective detection of microdeletions and inversions, and that it can only be performed on dividing cells.

Investigator responsibilities: 

1. Provide fixed, frozen, or actively growing cells. 
2. Complete a project application and CTA agreement form.
3. For human samples, provide a copy of an IRB letter of approval, if applicable.