G-Banding

G-banding is a staining technique in which alternating dark and light bands are produced on metaphase chromosomes, with each chromosome displaying a unique banding pattern. The chromosomes can then be counted and characterized to determine the karyotype of a cell. Some limitations of this technique are the ineffectiveness of determining small translocations, detecting microdeletions, and characterizing the chromosomes of cell lines which are complex. However, it is a fast and low-cost technique to determine chromosome number, aneuploidy, large translocations, and macrodeletions.

Investigator responsibilities: 

1. Provide fixed, frozen, or actively growing cells. 
2. Complete a project application and CTA agreement forms.
3. For human samples, provide a copy of an IRB letter of approval, if applicable.