G-banding is a staining technique in which alternating dark and light bands are produced on metaphase chromosomes, with each chromosome displaying a unique banding pattern. The chromosomes can then be counted and characterized to determine the karyotype of a cell. Some limitations of this technique are the ineffectiveness of determining small translocations, detecting microdeletions, and characterizing the chromosomes of cell lines which are complex. However, it is a fast and low-cost technique to determine chromosome number, aneuploidy, large translocations, and macrodeletions.
Investigator responsibilities:
- Provide fixed, frozen, or actively growing cells.
- Complete a project application.
- For human samples, provide a copy of an IRB letter of approval, if applicable.
- Van Andel Research Institute (VARI) requires a signed service agreement prior to initiating any project. The service agreement will be sent to you prior to starting the project. Once the agreement has been completed, signed and sent back to VARI, we will begin the project.
